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CD Trans® CHO Plus
适用于CHO-S等细胞系的复苏、传代及瞬时转染制备重组蛋白
SDS文件 产品手册 申请样品 订购咨询

Download Materials

资料下载

应用范围
  • 1. 适用于CHO-S细胞的高密度瞬转表达

    2. 转染后6-8天达到克级蛋白滴度峰值
    3. 单个细胞产率（Qp）较CHO-K1细胞瞬时转染提高约40%，达到E**iCHO表达系统3倍以上，取得CHO单克隆稳转细胞株Qp水平
    4. 采用专研FlashfectionTM极简转染工艺，省时，减小实验误差
    5. 在微型至摇瓶生物反应器中稳定放大

案例分享
“克级”蛋白快速制备
(A) 在50 mLTPP管中，以15 mL CHO-S细胞培养物表达10个分子。(B) 在125mL摇瓶中，以25 mL CHO-S细胞培养物表达5个分子。目标产物包含单克隆抗体（mAb）、人源Fc融合蛋白（FcFP）、双特异性抗体（BsAb）。转染时活细胞密度均为6×106 cells/mL。转染后第7天（Day7），CD Trans®CHO Plus Expression System中的目标分子产量显著高于ExpiCHO表达系统。与CD Trans®CHO Plus相比，转染后ExpiCHOTM中的细胞活率下降更为迅速。所有操作均严格依照各自生产厂商提供的标准流程。
FlashFection —简便、稳定转染
CodTrans可以使用FlashFection转染工艺。转染时，仅需直接将质粒与CodTrans依次加入细胞培养液。无需稀释→混合→控温孵育，即可获得与传统转染工艺一致的瞬转表达效果。FlashFection操作简便，适合高通量、自动化制备。
微型生物反应器至大体积摇瓶稳定放大
在从微型生物反应器放大至大体积摇瓶过程中，CDTrans®CHO Plus Expression System 维持了可比的产量。将CHO-S细胞培养至活细胞密度为6 × 10^6 cells/mL后进行转染，并持续补料至第5天。在初始细胞培养液为15mL/50 mL TPP管及180mL/1000mL摇瓶中，收获时（细胞活率<60%）的蛋白产率分别达到最优条件（25 mL/125 mL摇瓶）的68%和87%。
订购信息
订购咨询
类型 产品名称 规格 剂型 货号
试剂盒 CD Trans® CHO Plus Starter 1 kit(1L) 套装 AC602742-01
CD Trans® CHO Plus Expression System 1 kit(1L) 套装 AC607152-01
基础培养基 CD Trans® CHO Plus Medium 1000mL 液体 AC606165-01
补料培养基 CD Trans® CHO Plus Feed 1000mL 液体 AC606670-02
CD Trans® CHO Plus Feed 100mL 液体 AC606670-01
添加剂 CD Trans® CHO Plus Boost 100mL 液体 AC605778-02
CD Trans® CHO Plus Boost 10mL 液体 AC605778-01
CD Trans® CHO Plus Enhancer 100mL 液体 AC604943-02
CD Trans® CHO Plus Enhancer 10mL 液体 AC604943-01

稳转CHO平台

瞬转表达平台

疫苗平台

杂交瘤平台

经典培养基

细胞治疗平台

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Trial equipment application

试用装申请

申请样品类型
  • 稳转CHO平台
  • 转染试剂
  • 瞬转表达平台
  • CryoStasis™组织/细胞冻存液
  • 疫苗平台
  • 杂交瘤平台
  • 经典培养基
  • 细胞治疗平台
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